Part:BBa_K1891004:Experience

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Gene fragment of c-luciferase was amplified via PCR and verified by electrophoresis(Fig.1). The theoretic gene size of c-luciferase is 459bp, which matched our experimental results.

Fig.1 Electrophoresis result of c-luciferase gene fragments

Gene fragments were ligated to E.coli expression plasmid pET30a(+), after transformation, colony PCR was done to verify the efficiency(Fig.2A and 2B). Meanwhile, the sequencing results further confirmed that we successfully cloned the c-luciferase expression vectors.

Fig.2 Electrophoresis result of c-luciferase expression vectors
(A: electrophoresis result of colony PCR. The arrows show the correct sizes of α-tubulin, n-luciferase and c-luciferase.
B: electrophoresis result of colony PCR. The arrows show the correct size of β-tubulin.)

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